Polar Body Diagnosis for Hemophilia A Using Multiplex PCR for Linked Polymorphic Markers

Identification of Linked Markers for Delayed Fruit Ripening in Tomato Using Simple Sequence Repeat (SSR) Markers

Tomato (Solanum lycopersicum L.) is an important vegetable crop and acts as model plant for fruit development studies. Besides that, post-harvest damage is a devastating phenomenon often associated with ripening process in tomato which in turn leads to greater yield loss. Understanding the genetics, molecular and biochemical pathways is the key to overcome the existing situation. In th...

Messenger Rna Profiling: Body Identification Using Multiplex Real-time Pcr

Conventional methods of body fluid identification use labor-intensive, technologically diverse techniques that are performed in a series, not parallel, manner and are costly in terms of time and sample. Furthermore, for some frequently encountered body fluids no confirmatory technique exists. There is no definitive test, for example, for the presence of saliva or vaginal secretions. In seeking ...

mRNA Profiling: Body Fluid Identification Using Multiplex Real-Time PCR

Multiplex PCR protocol for the diagnosis of staphylococcal infection.

We report the development of a multiplex PCR protocol for the diagnosis of staphylococcal infection. The protocol was designed to (i) detect any staphylococcal species to the exclusion of other bacterial pathogens (based on primers corresponding to Staphylococcus-specific regions of the 16S rRNA genes), (ii) distinguish between S. aureus and the coagulase-negative staphylococci (CNS) (based on ...

Using blocks of linked single nucleotide polymorphisms as highly polymorphic genetic markers for parentage analysis.

Single nucleotide polymorphisms (SNPs) are plentiful in most genomes and amenable to high throughput genotyping, but they are not yet popular for parentage or paternity analysis. The markers are bi-allelic, so individually they contain little information about parentage, and in nonmodel organisms the process of identifying large numbers of unlinked SNPs can be daunting. We explore the possibili...